MAL62 overexpression enhances uridine diphosphoglucose-dependent trehalose synthesis and glycerol metabolism for cryoprotection of baker’s yeast in lean dough

Table 1 Characteristics of the strains used in the present study

Strains or plasmids	Relevant characteristics	Reference or source
Strains
E. coli DH5α	Φ80 lacZ M15ΔlacU169Δ recA1 endA1 hsdR17 supE44 thi-1 gyrA relA1	YCC
BY14a^a	MATa	YCC
BY14a + K	MATa, Yep-K	[15]
B + MAL62	MATa, Yep-PMK	[15]
B-T	MATa, tps1Δ:: KanMX	This study
B-T + M	MATa, tps1Δ:: MAL62	This study
B + TPS1	MATa, Yep-PTK	[16]
Plasmids
pUG6	E. coli/S. cerevisiae shuttle vector, containing Amp⁺, loxP-kanMX-loxP disruption cassette	[31]
pPGK1	bla LEU2 PGK1_P-PGK1_T	[58]

YCC: Yeast Collection Center of the Tianjin Key Laboratory of Industrial Microbiology
^aBY14a was selected as a high-leavening capacity haploid from 32 clones derived from BY14 (data not shown)

ISSN: 1475-2859