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Table 1 Characteristics of the strains used in the present study

From: MAL62 overexpression enhances uridine diphosphoglucose-dependent trehalose synthesis and glycerol metabolism for cryoprotection of baker’s yeast in lean dough

Strains or plasmids Relevant characteristics Reference or source
 E. coli DH5α Φ80 lacZ M15ΔlacU169Δ recA1 endA1 hsdR17 supE44 thi-1 gyrA relA1 YCC
 BY14aa MATa YCC
 BY14a + K MATa, Yep-K [15]
 B + MAL62 MATa, Yep-PMK [15]
 B-T MATa, tps1Δ:: KanMX This study
 B-T + M MATa, tps1Δ:: MAL62 This study
 B + TPS1 MATa, Yep-PTK [16]
 pUG6 E. coli/S. cerevisiae shuttle vector, containing Amp+, loxP-kanMX-loxP disruption cassette [31]
 pPGK1 bla LEU2 PGK1P-PGK1T [58]
  1. YCC: Yeast Collection Center of the Tianjin Key Laboratory of Industrial Microbiology
  2. aBY14a was selected as a high-leavening capacity haploid from 32 clones derived from BY14 (data not shown)