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Table 3 Kinetic and thermodynamic properties of afCR mutants

From: Improved thermostability of creatinase from Alcaligenes Faecalis through non-biased phylogenetic consensus-guided mutagenesis

Enzyme

Mutation

T5015 (°C)

Tm (°C)

M0

I304L/F395V

55.5 ± 0.1

59.6 ± 0.40

M1

M0 + D17V

59.06 ± 0.3

62.12 ± 0.02

M2-4

M1 + T199S

61.35 ± 0.2

63.93 ± 0.30

M3-4

M2-4 + L6P

62.21 ± 0.12

64.94 ± 0.20

M4-1

M3-4 + F108Y

61.0 ± 0.15

64.35 ± 0.31

M4-2

M3-4 + T251C

59.7 ± 0.1

64.24 ± 0.23

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Microbial Cell Factories

ISSN: 1475-2859

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