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Table 2 Bacterial strains and plasmids

From: Heterologous expression of AHL lactonase AiiK by Lactobacillus casei MCJΔ1 with great quorum quenching ability against Aeromonas hydrophila AH-1 and AH-4

Strain or plasmid

Description

Reference or source

Strains

 Kurthia huakuii LAM0618T

Wild type

ACCC 06121

 Escherichia coli DH5α

λф80dlacZΔM15 Δ (lacZYA-argF) U169 recA1 endA hsdR17 (r K m K ) supE44 thi-1 gyrA relA1

Tiangen

 Lactobacillus casei MCJΔ1

pMC11-cured strain

Chen et al. (2014) [27]

 Chromobacterium violaceum CV026

ATCC 31532 derivative, cviI::Tn5xylE Kmr, Smr

From Dr. Guishan Zhang

 Aeromonas hydrophila AH-1

Wild type

Isolated from dead grass carp

 Aeromonas hydrophila AH-4

Wild type

Isolated from dead grass carp

Plasmids

 pELX1

Expression vector, Ampr

Chen et al. (2014) [27]

 pUC55-NlpC

pUC55 containing NlpC gene

Constructed by BGI

 pELCW

Expression vector, Ampr

This study

 pELCW-aiiK

pELCW containing aiiK gene

This study