Fig. 5

Analysis of lra and rha gene expression. Relative abundances of lra and rha transcripts: a in wild type mycelia (AR5) 3 h after transfer to MM containing l-rhamnose as sole carbon source compared to transfer to MM containing lactose; b in the rhaR-deletion strain (AR225) compared to the rhaR⁺ isogenic control (AR271) 3 h after transfer of each to MM containing l-rhamnose; c in the isogenic wild type (AR5; creA⁺—unshaded box) and creA^d30 (AR305; shaded box) strains 3 h after transfer to inducing/repressing conditions compared to the corresponding gene and strain 3 h after transfer to inducing conditions; d in creA^d30 compared to wild type 3 h after transfer of each to l-rhamnose-containing medium. Box and whisker plots were generated using the program REST-2009 (Qiagen). The box areas of the plots encompass 50% of all observations, the dotted line represents the sample median and each whisker represents the outer 25% of observations. The Cp data used in these analyses were obtained from three independent biological replicates and RT-qPCR was done using three technical replicates. The genes encoding histone H2B (AN3469) and beta-tubulin benA (AN1182) were used as references for normalisation of expression. Primer pairs used were: lraA 398/399; lraB 404/405; lraC 346/347; rhaA 410/411; rhaE 352/353; H2B 394/395; benA 386/387