Fig. 3

Whole-cell biotransformation of epothilone C with engineered DSM 7029 to screen for EpoK-supporting ferredoxin reductases. a Schematic diagram: the whole-cell biotransformation system catalysed the conversion of epothilone C to epothilone A. b Epothilone C conversion rates of the fdx_0135 and fdx_S4580 overexpression strains at different reaction times (6 h, 12 h, 18 h, 24 h, and 30 h). Strain 7029-1 is DSM 7029 transformed with empty vector; strain H7029-2 is DSM 7029 with epoK expression. c Epothilone C conversion rates of strains overexpressing three ferredoxin reductase genes at different reaction times (6 h, 12 h, 18 h, 24 h, and 30 h). d Epothilone C conversion rates of strains overexpressing ferredoxin genes or ferredoxin reductase genes alone or in combination at different reaction times (6 h, 12 h, 18 h, 24 h, and 30 h). All strains above were analysed in triplicate, and the data are presented as the means ± standard deviations of the values from three independent experiments (n = 3)