Fig. 9

Purification by Ni-affinity chromatography of PfKch1¹⁻¹⁰⁹⁴-GFP and GFP-PfKch2^fl. The two channels were solubilized in FOS-12 + CHS and loaded on HisTrap Ni columns over night at 4 °C. The proteins were eluted from the HisTrap columns using a linear imidazole gradient from 10-500 mM (blue lines). Fluorescence was measured in each fraction and used to generate the elution profiles (green curves). a: Purification of PfKch1¹⁻¹⁰⁹⁴-GFP and b: GFP-PfKch2^fl. c: Coomassie Blue stain of the peak fractions from a and b separated in a 4–20% gradient Novex SDS-PAGE gel. Lanes 1: Mw marker 2:PfKch1^1-1094-GFP 3: GFP-PfKch2^fl and 4: Mw marker