Fig. 6
Bioreactor cultivation of the recombinant E. coli strains harboring CRISPRi system. a Fed-batch cultivation of the control strain E. coli BL21(DE3) carrying non-targeting CRISPRi vector pdCas9-none. b Fed-batch cultivation of the recombinant E. coli harboring CRISPRi vector pdCas9-pykF1icdA1 simultaneously targeting pykF and icdA genes. c Ratio variation in metabolites of the strain E. coli BL21(DE3) + pdCas9-pykF1icdA1 (CCRISPRi) versus control strain (Ccontrol) over time. Mean ± S.E. (n = 3)