Table 3 Applications of type V CRISPR/Cas systems in bacteria, including genome editing and transcriptional regulation
From: Application of different types of CRISPR/Cas-based systems in bacteria
Cas protein | Species | Strategy and type of modifications | Reference |
|---|---|---|---|
FnCas12a | C. glutamicum | Genome editing, 2 nucleotide substitutions 100% | [96] |
FnCas12a | E. coli | Genome editing, 3 heterologous genes were simultaneously inserted (20%) | [95] |
FnCas12a | S. coelicolor | Genome editing, knocked out (100%) | [93] |
AsCas12a | C. difficile | Multiple genome editing | [94] |
FndCas12a (D917A) | Y. lipolytica | CRISPRi (85%) | [100] |
AsdCas12a (E993A) | E. coli | Multiplex gene regulation | [98] |