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Table 2 Electrochemical assays at lipase based Biosensor

From: Microbial lipases and their industrial applications: a comprehensive review

Source of used lipase Analyte Principle of lipase use in assay Detection limit References
Electrochemical assays at lipase based biosensor
 Candida rugosa (Fungi) Methyl parathion
(p-nitrophenyl pesticides)
On a glass pH electrode lipase was mobilized and transformed which reduced the pH; methyl-paraoxon inhibit reaction 93 μmol/l [714]
 Burkholderia cepacia Lipase (Bacterium) Methyl parathion,
(p-nitrophenyl)
Lipase was immobilized on zeolitic nanoparticles and then into chitosan on a glassy carbon electrode, pesticides like methyl parathion were hydrolyzed to p-nitrophenyl that was electrochemically oxidized in the next ste 0.1–38 µM/l [715]
 Candida rugosa (Fungi) Diazinon Lipase converted diazinon to diethyl phosphorothioic acid and 2-isopropyl-4-methyl-6- hydroxypyrimidine. which caused a change in the impedance of the medium 10 nmol/l (fungal lipase) [306]
 Candida rugosa (Fungi) Chlorfenvinphos,
Malathion
Lipase converted p- nitrophenyl acetate to p- nitrophenol and acetic acid, p- nitrophenol was oxidized and a current at 0.024 V was recorded, analyzed inhibited lipase and stopped the reaction. 84.5 µmol/l for
chlorfenvinphos
and 282 µmol/l
for malathion
[716]
Optical assays-based on lipase biosensor
 Candida antarctica,
Yarrowia lipolytica and fungus
Lipase itself p-nitrophenyl butyrate
hydrolysis to butyric acid and p-nitrophenol, coloration caused by p-nitrophenol was measured
0.05 U/ml [717]
 Candida antarctica,
Mucor miehei,
Thermomyces lanuginosus (Fungus)
and bacteria Pseudomonas
cepacia and P. fluorescens
Lipase itself Butyryl 4-methyl umbelliferone (Bu-4-Mu) and methanol in tert-butanol were trans-esterified in the presence of lipase, production of 4-methylumbelliferone was measured fluorometre Not available [718]