From: Microbial lipases and their industrial applications: a comprehensive review
Source of used lipase | Analyte | Principle of lipase use in assay | Detection limit | References |
---|---|---|---|---|
Electrochemical assays at lipase based biosensor | ||||
 Candida rugosa (Fungi) | Methyl parathion (p-nitrophenyl pesticides) | On a glass pH electrode lipase was mobilized and transformed which reduced the pH; methyl-paraoxon inhibit reaction | 93 μmol/l | [714] |
 Burkholderia cepacia Lipase (Bacterium) | Methyl parathion, (p-nitrophenyl) | Lipase was immobilized on zeolitic nanoparticles and then into chitosan on a glassy carbon electrode, pesticides like methyl parathion were hydrolyzed to p-nitrophenyl that was electrochemically oxidized in the next ste | 0.1–38 µM/l | [715] |
 Candida rugosa (Fungi) | Diazinon | Lipase converted diazinon to diethyl phosphorothioic acid and 2-isopropyl-4-methyl-6- hydroxypyrimidine. which caused a change in the impedance of the medium | 10 nmol/l (fungal lipase) | [306] |
 Candida rugosa (Fungi) | Chlorfenvinphos, Malathion | Lipase converted p- nitrophenyl acetate to p- nitrophenol and acetic acid, p- nitrophenol was oxidized and a current at 0.024 V was recorded, analyzed inhibited lipase and stopped the reaction. | 84.5 µmol/l for chlorfenvinphos and 282 µmol/l for malathion | [716] |
Optical assays-based on lipase biosensor | ||||
 Candida antarctica, Yarrowia lipolytica and fungus | Lipase itself | p-nitrophenyl butyrate hydrolysis to butyric acid and p-nitrophenol, coloration caused by p-nitrophenol was measured | 0.05 U/ml | [717] |
 Candida antarctica, Mucor miehei, Thermomyces lanuginosus (Fungus) and bacteria Pseudomonas cepacia and P. fluorescens | Lipase itself | Butyryl 4-methyl umbelliferone (Bu-4-Mu) and methanol in tert-butanol were trans-esterified in the presence of lipase, production of 4-methylumbelliferone was measured fluorometre | Not available | [718] |