Fig. 6
Genetic information can be recovered from a single HC-PCAM. a LB agar plate showing transformants after electroporation of isolate from a single microcapsule into E. coli cells. b PCR amplification of the SrtF gene from colonized (C) and empty (E) microcapsules taken before (CB and EB) and after (CA and EA) the sortase-mediated conjugation, showing that the plasmid is not significantly exchanged between microcapsules. NC: Negative control (No template), PC positive control, M DNA ladder, C colonized microcapsule