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Table 4 Strains and plasmids used in this study

From: Metabolic engineering of Escherichia coli for l-malate production anaerobically

Strains or plasmids Relevant description Sources
Strains
 BA002 E. coli K12, ΔpflB, ΔldhA Previous work
 BA040 BA002, ΔfumB, ΔfrdABCD Previous work
 BA041 BA040/pTrc99a-mdh This study
 BA042 BA040/pTrc99a-pncB This study
 BA050 BA040, ΔptsG This study
 BA051 BA050/pTrc99a-ppc This study
 BA052 BA050/pTrc99a-pyc This study
 BA060 BA050, Δppc This study
 BA061 BA060/pTrc99a-Bspck This study
 BA062 BA060/pTrc99a-Bspck-pncB This study
 BA063 BA060/pTrc99a-Bspck-mdh This study
Plasmids
 pMD19-T T-vector; Apr TAKARA
 pTrc99a Expression vector with Trc promoter; Apr BioVector
 pKD46 Vector containing Red (γ, β, and exo) recombination functions under control of ParaB promote, Apr CGSC
 pTrc99a-mdh mdh gene from E. coli K12 Previous work
 pTrc99a-pncB pncB gene from E. coli K12 Previous work
 pTrc99a-ppc ppc gene from E. coli K12 Previous work
 pTrc99a-pyc pyc gene from Lactococcus lactis subsp. cremoris NZ9000 Previous work
 pTrc99a-Bspck pck gene from Bacillus subtilis 168 Previous work
 pTrc99a- Bspck-mdh pck gene from Bacillus subtilis 168 and mdh gene from E. coli K12 This study
 pTrc99a-Bspck-pncB pck gene from Bacillus subtilis 168 and pncB gene from E. coli K12 This study