Table 4 Strains and plasmids used in this study
From: Metabolic engineering of Escherichia coli for l-malate production anaerobically
Strains or plasmids | Relevant description | Sources |
|---|---|---|
Strains | ||
BA002 | E. coli K12, ΔpflB, ΔldhA | Previous work |
BA040 | BA002, ΔfumB, ΔfrdABCD | Previous work |
BA041 | BA040/pTrc99a-mdh | This study |
BA042 | BA040/pTrc99a-pncB | This study |
BA050 | BA040, ΔptsG | This study |
BA051 | BA050/pTrc99a-ppc | This study |
BA052 | BA050/pTrc99a-pyc | This study |
BA060 | BA050, Δppc | This study |
BA061 | BA060/pTrc99a-Bspck | This study |
BA062 | BA060/pTrc99a-Bspck-pncB | This study |
BA063 | BA060/pTrc99a-Bspck-mdh | This study |
Plasmids | ||
pMD19-T | T-vector; Apr | TAKARA |
pTrc99a | Expression vector with Trc promoter; Apr | BioVector |
pKD46 | Vector containing Red (γ, β, and exo) recombination functions under control of ParaB promote, Apr | CGSC |
pTrc99a-mdh | mdh gene from E. coli K12 | Previous work |
pTrc99a-pncB | pncB gene from E. coli K12 | Previous work |
pTrc99a-ppc | ppc gene from E. coli K12 | Previous work |
pTrc99a-pyc | pyc gene from Lactococcus lactis subsp. cremoris NZ9000 | Previous work |
pTrc99a-Bspck | pck gene from Bacillus subtilis 168 | Previous work |
pTrc99a- Bspck-mdh | pck gene from Bacillus subtilis 168 and mdh gene from E. coli K12 | This study |
pTrc99a-Bspck-pncB | pck gene from Bacillus subtilis 168 and pncB gene from E. coli K12 | This study |