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Table 2 Intracellular NAD(H) and ATP concentration during in anaerobic phase at 48 h

From: Metabolic engineering of Escherichia coli for l-malate production anaerobically

Strain NADH (µmol/g DCWa) NAD+ (µmol/g DCW) Total NAD(H) (µmol/g DCW) NADH/NAD+ ATP (nmol/g DCW)
BA040 6.16 ± 0.12 9.47 ± 0.20 15.63 ± 0.34 0.65 ± 0.02 338.89 ± 10.84
BA041 5.93 ± 0.41 11.57 ± 0.11 17.50 ± 0.17 0.51 ± 0.01 362.45 ± 12.55
BA042 10.75 ± 0.62 17.65 ± 0.55 28.40 ± 0.42 0.61 ± 0.01 388.85 ± 16.64
BA050 4.85 ± 0.22 9.14 ± 0.28 13.99 ± 0.65 0.53 ± 0.01 358.84 ± 15.82
BA051 5.85 ± 0.05 10.86 ± 0.25 16.71 ± 0.15 0.53 ± 0.02 385.65 ± 18.85
BA052 5.93 ± 0.01 11.57 ± 0.11 17.50 ± 0.16 0.51 ± 0.01 354.25 ± 12.46
BA061 14.04 ± 0.74 16.73 ± 0.86 30.77 ± 0.53 0.84 ± 0.02 667.43 ± 23.87
BA062 17.71 ± 0.43 20.56 ± 0.32 38.27 ± 0.93 0.86 ± 0.03 683.26 ± 27.55
BA063 16.33 ± 0.51 22.52 ± 0.52 38.85 ± 0.37 0.72 ± 0.02 715.79 ± 25.78
  1. Each value is the mean of three parallel replicates ± standard deviation
  2. a DCW, dry cell weight