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Table 2 Intracellular NAD(H) and ATP concentration during in anaerobic phase at 48 h

From: Metabolic engineering of Escherichia coli for l-malate production anaerobically

Strain

NADH (µmol/g DCWa)

NAD+ (µmol/g DCW)

Total NAD(H) (µmol/g DCW)

NADH/NAD+

ATP (nmol/g DCW)

BA040

6.16 ± 0.12

9.47 ± 0.20

15.63 ± 0.34

0.65 ± 0.02

338.89 ± 10.84

BA041

5.93 ± 0.41

11.57 ± 0.11

17.50 ± 0.17

0.51 ± 0.01

362.45 ± 12.55

BA042

10.75 ± 0.62

17.65 ± 0.55

28.40 ± 0.42

0.61 ± 0.01

388.85 ± 16.64

BA050

4.85 ± 0.22

9.14 ± 0.28

13.99 ± 0.65

0.53 ± 0.01

358.84 ± 15.82

BA051

5.85 ± 0.05

10.86 ± 0.25

16.71 ± 0.15

0.53 ± 0.02

385.65 ± 18.85

BA052

5.93 ± 0.01

11.57 ± 0.11

17.50 ± 0.16

0.51 ± 0.01

354.25 ± 12.46

BA061

14.04 ± 0.74

16.73 ± 0.86

30.77 ± 0.53

0.84 ± 0.02

667.43 ± 23.87

BA062

17.71 ± 0.43

20.56 ± 0.32

38.27 ± 0.93

0.86 ± 0.03

683.26 ± 27.55

BA063

16.33 ± 0.51

22.52 ± 0.52

38.85 ± 0.37

0.72 ± 0.02

715.79 ± 25.78

  1. Each value is the mean of three parallel replicates ± standard deviation
  2. a DCW, dry cell weight