Fig. 7

Glucose yield (a) and xylose yield (b) during hydrolysis of alkali pretreated wheat straw. The hydrolysis reactions were performed with 2% (w/v) DA-WS for 120 h. M. cinnamomea N12 enzymes and Celluclast 1.5L were added at 10 and 20 mg enzyme/g dry substrate, respectively. In separate hydrolysis DA-WS was hydrolyzed at 50 °C by M. cinnamomea enzymes (ME) or Celluclast 1.5L (CE) alone. In simultaneous hydrolysis (SIH) DA-WS was co-hydrolyzed by the enzyme of M. cinnamomea N12 and Celluclast 1.5L at 50 °C for 120 h. In sequential hydrolysis (SEH) DA-WS was initially hydrolyzed by M. cinnamomea N12 enzymes at 65 °C for 24 h and continuously incubated at 50 °C for 96 h after the addition of Celluclast 1.5L. CE commercial cellulases, ME M. cinnamomea N12 enzymes, SIH simultaneous hydrolysis, SEH sequential hydrolysis. The mean values of three replicates and standard deviations were presented