A novel knock out strategy to enhance recombinant protein expression in Escherichia coli

Table 3 Asparaginase quantitation in supernatant of 20 h post-induction culture of the double knock-out, single knock-outs and control strain

Gene knock-out Strains	Culture OD₆₀₀	Asparaginase Assay (OD₄₃₆)	Asparaginase units/ml	Asparaginase mg/ml
∆(elaA + cysW)	7.87	0.7905	70.39	0.35
∆(elaA + cueR)	7.52	0.7755	69.50	0.35
∆(cysW + purL)	5.49	0.5975	53.46	0.27
∆(yabI + cysW)	7.92	0.496	44.55	0.22
∆purL	9.10	0.514	46.33	0.23
∆elaA	12.19	0.196	17.82	0.09
∆cysW	8.50	0.495	44.55	0.22
∆cueR	7.78	0.473	41.88	0.21
∆cysJ	7.67	0.3745	32.97	0.16
∆yfbN	13.20	0.355	32.08	0.16
BW25113	8.65	0.336	30.29	0.15

ISSN: 1475-2859