Table 3 Strains and plasmids used in this study

Strains

L. plantarum

CGMCC 0847

 ST-III

Wild type

 ΔsacR1::cat

Derivative of ST-III containing a lox66-P32-cat-lox71 replacement of sacR1

This study

 ΔsacR2::cat

Derivative of ST-III containing a lox66-P32-cat-lox71 replacement of sacR2

This study

 ΔsacR1

Derivative of ST-III containing a lox72 replacement of sacR1

This study

 ΔsacR2

Derivative of ST-III containing a lox72 replacement of sacR2

This study

 409-Flag-sacR1

Derivative of ST-III harboring pSIP409-Flag-sacR1

This study

 409-Flag-sacR2

Derivative of ST-III harboring pSIP409-Flag-sacR2

This study

E. coli

 DH5α

For general gene cloning and plasmid construction

Promega

 BL21

For protein expression

Novagen

 BL21-sacR1

E. coli BL21 (DE3) harboring pTolo-EX5-sacR1

This study

 BL21-sacR2

E. coli BL21 (DE3) harboring Pet28a-sacR2

This study

Plasmid

 pTolo-EX5

Ap^R, for cloning and protein expression, included His-tag

Tolobio

 pET-28a ( +)

Kana^R, for cloning and protein expression, included His-tag

Novagen

 pTolo-EX5-sacR1

Ap^R, pTolo-EX5 with sacR1 gene cloned into XhoI sites

This study

 pET-28-sacR2

Kana^R, pET-28a ( +) with sacR2 gene cloned into NheI/HindШ sites

This study

 pNZ5319

Cm^R, Em^R; for multiple gene replacements in Gram-positive bacteria

[29]

 pNZ5319-up-down-1

Cm^R, Em^R; pNZ5319 derivative containing homologous regions up and downstream of sacR1

This study

 pNZ5319-up-down-2

Cm^R, Em^R; pNZ5319 derivative containing homologous regions up and downstream of sacR2

This study

 pNZ5348

Em^R; contains cre under the control of the lp_1144 promoter

[29]

 pSIP409

Em^R; for shuttle vector in E.coil, gusA controlled by P_sppQ

[53]

 pSIP409-Flag-sacR1

Em^R; pSIP409 derivative; gusA replaced by Flag-tagged sacR1

This study

 pSIP409-Flag-sacR2

Em^R; pSIP409 derivative; gusA replaced by Flag-tagged sacR2

This study