From: Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation
Factor
Name
Units
Type
Level
Minimum (−)
Maximum (+ 1)
A
Temperature
°C
Numeric
25
30
B
pH
–
6
8
C
Agitation
RPM
100
180
D
NH4NO3 conc
%
0.1
1
E
Yeast Extract
0
0.2
F
NaCl conc
0.5