Fig. 3
From: Nucleic acids in inclusion bodies obtained from E. coli cells expressing human interferon-gamma
a Agarose gel-electrophoresis of nucleic acids isolated from purified hIFNγ IBs. 1—Sample, isolated from IBs, b Agarose gel-electrophoresis of nucleic acids isolated from purified hIFNγ IBs treated with restriction endonuclease XhoI and c HindIII. 1—Non-treated sample; 2(b)—Sample treated with XhoI; 2(c)—sample treated with HindIII; d Enzymatic digestion of nucleic acids isolated from purified hIFNγ IBs 1—non treated sample; 2—sample treated with mixture of RNase A and RNase T1; 3—sample treated with DNase I; 4—sample treated with Proteinase K; M—Molecular weight marker, bp