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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Expression, homology modeling and enzymatic characterization of a new β-mannanase belonging to glycoside hydrolase family 1 from Enterobacter aerogenes B19

Fig. 2

Homology modeling and the active center of Man1E from Enterobacter aerogenes B19. a The modelled 3D-structure of Man1E based on Rhizomucor miehei Man5A. Blue indicated N- terminus, red indicated C- terminus. b Vacuum electrostatic surface of Man1E. c Ramachandran plot of Man1E homologous model structure. Red area indicated most favoured regions, yellow area indicated additional allowed regions, faint yellow area indicated generously allowed regions, white area indicated disallowed regions. d-e The active center of Man1E predicted by bSiteFinder. Overlap of Man1E and PaMan5A (PDB code, 3ZIZ) (d). yellow represents Man1E, cyan represents PaMan5A. The amino acid residues linked to the ligand in the predicted catalytic center (e). TRS represents 2-amino-2-hydroxymethyl-propane-1,3-diol

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Microbial Cell Factories

ISSN: 1475-2859

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