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Table 1 Catalytic constants of α-glucosidases from M. gruessii and M. reukaufii

From: Molecular characterization and heterologous expression of two α-glucosidases from Metschnikowia spp, both producers of honey sugars

Substrate

Sucrose

Maltose

Enzyme

Km (mM)

kcat (s−1)

kcat/Km (s−1mM−1)

Km (mM)

kcat (s−1)

kcat/Km (s−1mM−1)

Mg-αGlu

54.1 ± 5.8

269.7 ± 10.9

4.9 ± 0.7

42.8 ± 5.6

365.6 ± 16.7

8.5 ± 1.5

Mr-αGlu

64.9 ± 4.6

477.2 ± 13.7

7.4 ± 0.7

79.8 ± 12.8

405.9 ± 28.1

5.1 ± 1.2

  1. Reaction rates measurements were performed by triplicate. Values of kcat were calculated from Vmax considering the theoretical molecular mass of 66 kDa. Standard errors are indicated