Table 2 Effect of protease inhibitors and denaturing agents on the enzymatic activity of NHSSP
Reagent | Concentration | Relative activity (%) |
|---|---|---|
none | – | 100 |
SDS | 1% | 8 |
Triton | 1% | 98 |
Urea | 4 M | 95 |
DTT | 5 mM | 105 |
β-Mercaptoethanol | 5 mM | 99 |
PMSF | 1 mM | 40 |
EDTA | 1 mM | 100 |
TCEP | 1 mM | 97 |