From: An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing
Reagent
Concentration
Relative activity (%)
none
–
100
SDS
1%
8
Triton
98
Urea
4 M
95
DTT
5 mM
105
β-Mercaptoethanol
99
PMSF
1 mM
40
EDTA
TCEP
97