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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Development of a new gene expression vector for Thermus thermophilus using a silica-inducible promoter

Fig. 3

SDS-PAGE and Western blot analysis of recombinant proteins produced in T. thermophilus and E. coli. Left: Expression of His-tagged β-galactosidase in T. thermophilus HB27 in response to 0 and 10 mM silicic acid. Right: Expression of recombinant β-galactosidase in E. coli. Cells were harvested by centrifugation and disrupted by sonication. The soluble fraction was separated from insoluble fractions by centrifugation. Each lane contains 10 µg of total protein. His-tagged proteins were detected using anti-His-tag mAb-HRP-DirecT

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Microbial Cell Factories

ISSN: 1475-2859

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