Fig. 6From: Saccharomyces cerevisiae nutrient signaling pathways show an unexpected early activation pattern during winemakingWestern blot analysis of Rps6 phosphorylation along fermentation using an alternative protein extraction method. Strains EC1118 and BQS252 were grown in synthetic grape juice MS300 and MS60 and cellular extracts were obtained by mechanical breakage using glass beads. Specific antibodies against Rps6 phosphorylated and total forms were usedBack to article page