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Table 2 Comparison of Iegfp and egfp gene in mRNA level

From: The signal peptide of Cry1Ia can improve the expression of eGFP or mCherry in Escherichia coli and Bacillus thuringiensis and enhance the host’s fluorescent intensity

No. TAc-eGFP TAc-IeGFP Fold change
Mean Ct of hcat gene Mean Ct of egfp gene Mean of 2−ΔCt Variable coefficient (%) Mean Ct of hcat gene Mean Ct of Iegfp gene Mean of 2−ΔCt Variable coefficient (%)
8 h
 1 26.91 ± 0.159 13.82 ± 0.131 989.466 ± 1018.333 10.289 30.18 ± 0.162 14.11 ± 0.118 75,603.881 ± 9462.533 12.516 7.639
 2 27.12 ± 0.024 13.78 ± 0.200 30.42 ± 0.031 14.30 ± 0.153
 3 27.40 ± 0.076 14.03 ± 0.476 27.94 ± 0.033 11.54 ± 0.292
12 h
 1 26.59 ± 0.136 14.73 ± 0.203 2945.768 ± 980.636 33.290 27.29 ± 0.088 11.86 ± 0.079 38,480.124 ± 14,483.546 37.639 13.639
 2 27.21 ± 0.111 15.53 ± 0.353 26.68 ± 0.128 12.25 ± 0.120
 3 26.30 ± 0.058 15.46 ± 0.199 25.55 ± 0.086 9.97 ± 0.065
  1. The precision of the measures was indicated by the standard deviations