The signal peptide of Cry1Ia can improve the expression of eGFP or mCherry in Escherichia coli and Bacillus thuringiensis and enhance the host’s fluorescent intensity

Table 2 Comparison of Iegfp and egfp gene in mRNA level

No.	TAc-eGFP				TAc-IeGFP				Fold change
No.	Mean Ct of hcat gene	Mean Ct of egfp gene	Mean of 2^−ΔCt	Variable coefficient (%)	Mean Ct of hcat gene	Mean Ct of Iegfp gene	Mean of 2^−ΔCt	Variable coefficient (%)	Fold change
8 h
1	26.91 ± 0.159	13.82 ± 0.131	989.466 ± 1018.333	10.289	30.18 ± 0.162	14.11 ± 0.118	75,603.881 ± 9462.533	12.516	7.639
2	27.12 ± 0.024	13.78 ± 0.200			30.42 ± 0.031	14.30 ± 0.153
3	27.40 ± 0.076	14.03 ± 0.476			27.94 ± 0.033	11.54 ± 0.292
12 h
1	26.59 ± 0.136	14.73 ± 0.203	2945.768 ± 980.636	33.290	27.29 ± 0.088	11.86 ± 0.079	38,480.124 ± 14,483.546	37.639	13.639
2	27.21 ± 0.111	15.53 ± 0.353			26.68 ± 0.128	12.25 ± 0.120
3	26.30 ± 0.058	15.46 ± 0.199			25.55 ± 0.086	9.97 ± 0.065

ISSN: 1475-2859