Fig. 2
Expression analysis of eGFP and IeGFP regulated by Pac promoter in E.coli TG1 strain. a Lane “-” is the negative control which prepared from T304 cells sampled at 12 h after inoculation. TAc-IeGFP and TAc-eGFP cells were separately taken at 4, 8, 10, 12 and 24 h after inoculation. Lane “M” is the molecular weight standards. b Comparison of the collected cells when they were incubated for 24 h. c The fluorescent intensities and their fold changes for TAc-IeGFP over TAc-eGFP strains at corresponding sampling time. The slit widths of EX/EM were 3 nm and 5 nm respectively and the detections were conducted in low sensitivity. The fluorescent signals of T304 cells cannot be detected. The error bars indicate standard error of mean. The significant differences of the fluorescent intensities between TAc-IeGFP and TAc-eGFP cells at corresponding time were indicated by single asterisk (p < 0.05) or double asterisks (p < 0.01)