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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Enhanced production of recombinant proteins in Corynebacterium glutamicum by constructing a bicistronic gene expression system

Fig. 2

EGFP expression level of the BCD expression vectors. pXMJ19-EGFP and pXMJ19-0 were used as control and control check (CK). a The fluorescence intensity of BCD expression vectors contained different fore-cistron sequences in C. glutamicum. b The fluorescence intensity of enhanced BCD expression vectors in E. coli. c The SDS-PAGE analysis of EGFP expression of the top three BCD expression vectors. Lane M: Protein Marker 26616; lane1-3: pbtac-HT-8-EGFP, pbtac-HT-11-EGFP, pbtac-HP-9-EGFP; lane4: pXMJ19-EGFP; lane5: CK, pXMJ19 without the EGFP gene. d The fluorescence intensity and growth curve of the top three strongest BCD expression vectors at different time points

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Microbial Cell Factories

ISSN: 1475-2859

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