Fig. 5
From: Metabolic engineering of Escherichia coli for efficient production of l-alanyl-l-glutamine
Production of AQ by engineered strains co-expressing CgGlnA and BsBacD. WT wild type; BW, AQ02, AQ04, and AQ06 were transformed with plasmid p11. The engineered strains were induced and suspended in a reaction mixture containing 100 mM sodium glutamate, 100 mM alanine, 200 mM ammonium chloride, and 10 mM magnesium chloride. The bioconversion reactions were performed at 30 °C and 200 rpm for 18 h. Glucose was supplemented at a concentration of 10 mM every 3 h