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Table 2 Comparison of the antimicrobial activity of cell-free supernatants (CFSs) of Lb. plantarum SF9C and Lb. brevis SF9B, separately and combined, before and after the treatment with proteinase K and high temperature heating, against L. monocytogenes ATCC® 19111™, S. aureus 3048, E. coli 3014 and S. Typhimurium FP1, determined by agar well-diffusion assay and expressed as the diameter of inhibition zones around the wells (cm)

From: The functional capacity of plantaricin-producing Lactobacillus plantarum SF9C and S-layer-carrying Lactobacillus brevis SF9B to withstand gastrointestinal transit

Treatment of CFS

Lactobacillus strains

Diameter of inhibition zone (cm)

L. monocytogenes ATCC® 19111™

S. aureus 3048

E. coli 3014

S. Typhimurium FP1

Before treatment

Lb. plantarum SF9C

1.92 ± 0.03az

1.57 ± 0.06ay

1.50 ± 0.05ayw

1.38 ± 0.03aw

Lb. brevis SF9B

0.00 ± 0.00ez

0.00 ± 0.00ez

0.00 ± 0.00ez

0.00 ± 0.00fz

Combined SF9C + SF9B

1.72 ± 0.03bz

0.98 ± 0.03dx

1.13 ± 0.06cy

1.00 ± 0.00cx

proteinase K

Lb. plantarum SF9C

1.10 ± 0.00dy

1.17 ± 0.03cyz

1.23 ± 0.03bz

1.20 ± 0.00bxz

Lb. brevis SF9B

0.00 ± 0.00ez

0.00 ± 0.00ez

0.00 ± 0.00ez

0.00 ± 0.00fz

Combined SF9C + SF9B

1.02 ± 0.03dz

0.90 ± 0.00dy

0.98 ± 0.03dz

0.85 ± 0.00ey

100 °C/30 min

Lb. plantarum SF9C

1.68 ± 0.03bz

1.52 ± 0.03by

1.30 ± 0.00bx

1.23 ± 0.03bx

Lb. brevis SF9B

0.00 ± 0.00ez

0.00 ± 0.00ez

0.00 ± 0.00ez

0.00 ± 0.00fz

Combined SF9C + SF9B

1.53 ± 0.06cz

0.93 ± 0.06dx

1.03 ± 0.06cdy

0.92 ± 0.03dx

  1. abcdefDifferent letter means statistically significant difference (p < 0.05) within the same column among the treatments of CFSs and used Lactobacillus strains
  2. wxyzDifferent letter means statistically significant difference (p < 0.05) within the same row among the used pathogens. Statistical analysis was carried out using ANOVA and the results are reported as mean value ± SD of three independent experiments