Strain/plasmid | Relevant characteristics | References |
---|---|---|
E. coli DH5α | Host for plasmid cloning | Invitrogen |
ATCC 13032 | C. glutamicum wild type Biotin auxotrophic | ATCCa |
CGR6 | ATCC13032∆pta∆ackA∆ldh∆butA∆nagD, ∆ackA::Ptuf -alsSD | [9] |
CGS1 | CGR6 ∆ppc | This study |
CGS2 | CGR6 ∆pyc | This study |
CGS3 | CGR6 ∆ppc∆pyc | This study |
CGS4 | CGS3 ICDA94D | This study |
CGS5 | CGS3 ICDG407S | This study |
CGS6 | CGS3 ICDR453S | This study |
CGS7 | CGR6 P1-gltA | This study |
CGS8 | CGS1 P1-gltA | This study |
CGS9 | CGS8 ∆butA::Ptuf -alsSD, ∆nagD::Ptuf -alsSD | This study |
CGS11 | CGS9 pEC-XK99E-alsSD-∆lacIq | This study |
Plasmids | ||
 pD-sacB | KanR; vector for in-frame deletion (sacBB.sub.; lacZα; OriVE.c.) | [56] |
 pD-sacB-ppc | pD-sacB carrying the flanking sequences of the ppc gene | This study |
 pD-sacB-pyc | pD-sacB carrying the flanking sequences of the pyc gene | This study |
 pD-sacB-butA | pD-sacB carrying the flanking sequences of the butA gene | [41] |
 pD-sacB-nagD | pD-sacB carrying the flanking sequences of the nagD gene | [9] |
 pD-sacB-icd-mut-1 | KanR, containing the nucleotide sequence for ICD amino acid exchange A94D | This study |
 pD-sacB-icd-mut-2 | KanR, containing the nucleotide sequence for ICD amino acid exchange G407S | This study |
 pD-sacB-icd-mut-3 | KanR, containing the nucleotide sequence for ICD amino acid exchange R453S | This study |
 pD-sacB-P1-gltA | KanR, containing P1 promoter and gltA flanks | Unpublished work |
 pD-sacB-butA-alsSD | KanR, containing Ptuf -alsSD flanks | This study |
 pD-sacB-nagD-alsSD | KanR, containing Ptuf -alsSD flanks | This study |
 pEC-XK99E | KanR; C. glutamicum/E. coli shuttle vector (Ptrc, lacIq; pGA1, OriVC.g., OriVE.c.) | [57] |
 pEC-XK99E-alsSD-ΔlacIq | Derived from pEC-XK99E, for the overexpression of alsS and alsD under promoter Ptrc | [9] |