Table 2 Key primers used in this study
Primer | Sequence 5′-3′ | Purpose |
|---|---|---|
BBP950 | GAGCACACCCATCACGGTCAGAG | nifLA modification confirmation |
BBP1322 | GATCTCCATCGACTCGATCTTGTCCAGGGTGAAC | nifLA modification confirmation |
BBP2006 | CACGTGCCAGGAATTCCTCCATG | amtB gene deletion confirmation |
BBP2007 | CTGTGGACGATGGCCAGGGACATGGATC | amtB gene deletion confirmation |