Fig. 3

In vivo recombination of heterologous modules. a Each fragments crtZ/crtW carry an Ura3/His3 marker and two homologous arms with Ty1 (Ty-1, Ty-2). All crtZ and crtW fragments were mixed up to transform into yQDD001. The fragments inserted into the Ty1 sites of yeast genome randomly to produce the yeast library with various combinations of crtZ and crtW. b Yeast colonies of in vivo recombination method screened in the selected medium. The yeast library contained various yeast with different colors and sizes. c Astaxanthin yield measurement of in vivo recombination evolved strains. The genotype of yQDD011–yQDD022 were proved by PCRTag analysis