Co-production of gallic acid and a novel cell-associated tannase by a pigment-producing yeast, Sporidiobolus ruineniae A45.2

Table 2 Experimental design matrix of PBD and response variables for screening of the most significant factors affecting co-production of gallic acid, CAT and viable cell numbers

Run	A: Tannic acid (g/L)	B: Yeast (g/L)	C: Glucose (g/L)	D: (NH₄)₂SO₄ (g/L)	E: Tween80 (g/L)	F: Glutamate (g/L)	G: Time (h)	Gallic acid (g/L)	CAT (mU/mL)	Viable cell numbers (logCFU/mL)
1	5	10	2	10	2	5	24	3.50	18.08	7.35
2	1	10	10	2	2	5	24	0.23	3.81	7.58
3	5	2	10	10	0.5	5	72	3.82	15.99	6.32
4	1	10	2	10	2	1	72	0.18	0.00	4.70
5	1	2	10	2	2	5	72	0.34	2.95	7.22
6	1	2	2	10	0.5	5	24	1.09	13.56	6.96
7	5	2	2	2	2	1	72	4.42	18.32	7.37
8	5	10	2	2	0.5	5	72	4.99	19.70	7.45
9	5	10	10	2	0.5	1	24	4.85	16.91	7.16
10	1	10	10	10	0.5	1	72	0.46	5.32	6.64
11	5	2	10	10	2	1	24	2.95	12.99	7.11
12	1	2	2	2	0.5	1	24	1.06	13.55	7.08
13	3	6	6	6	1.25	3	48	2.57	18.71	7.87
14	3	6	6	6	1.25	3	48	2.65	19.68	7.93
15	3	6	6	6	1.25	3	48	2.64	16.68	7.75

ISSN: 1475-2859