Fig. 3

DHA content (% DCW, w/w) in repeated fed-batch cultivation of two and four cycles. a DHA content of two cycles under different glucose concentrations. The DHA content in cycle 1 was set as 100% under different glucose concentrations respectively, and the percentage changes in cycle 2 were shown; b DHA content of two cycles under different medium replacement ratios. The DHA content in cycle 2 under medium replacement ratio of 90% was set as 100%, and the percentage changes in cycle 2 under medium replacement ratio of 80% and 70% were shown; c DHA content of four cycles. The DHA content in cycle 1 was set as 100%, and the percentage changes in other cycles were shown. Asterisks indicated significant differences, as evaluated by a statistical Student’s T-test (*p < 0.05, **p < 0.01, ***p < 0.001)