Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae

Zhang, Zhongxi; Yang, Yang; Wang, Yike; Gu, Jinjie; Lu, Xiyang; Liao, Xianyan; Shi, Jiping; Kim, Chul Ho; Lye, Gary; Baganz, Frank; Hao, Jian

doi:10.1186/s12934-020-01347-8

Microbial Cell Factories

Table 2 Strains and plasmids

From: Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae

Strain or plasmid	Relevant genotype and description	Reference or source
K. pneumoniae Δgad	Δgad	[7]
E. coli W3110	Wild type	Lab stock
E. coli BL21/yqhD	Over-expression of yqhD	This work
E. coli BL21/aldB	Over-expression of aldB	This work
E. coli BL21/betB	Over-expression of betB	This work
E. coli BL21/ad1	Over-expression of ad1	This work
E. coli BL21/ad2	Over-expression of ad2	This work
Enterobacter cloacae S1	Wild type,	This work
E. cloacae ΔyjhG	ΔyjhG, Str^r	This work
E. cloacae ΔyjhH	ΔyjhH, Apr^r	This work
E. cloacae ΔyhcH	ΔyhcH, Apr^r	This work
E. cloacae ΔyagG	ΔyagG, Apr^r	This work
E. cloacae ΔiclR	ΔiclR, Apr^r	This work
E. cloacae ΔxyL	Δxyl, Apr^r	This work
E. cloacae ΔyqhD	ΔyqhD, Str^r	This work
E. cloacae ΔbetB	ΔbetB, Str^r	This work
E. cloacae +yqhD	pSARI-yqhD, Kan^r	This work
E. cloacae +betB	pSARI-betB, Kan^r	This work
pMD18-T-simple	Amp^r, TA cloning vector, 2692 bp	Takara^®
pMD18T-yhcH	Amp^r, carries yhcH, 4,237 bp	This work
pMD18T-ΔyhcH	Amp^r, carries part of yhcH, Apr^r, 5077 bp	This work
pMD18T-yjhH	Amp^r, carries yjhH, 4,844 bp	This work
pMD18T-ΔyjhH	Amp^r, carries part of yjhH, Apr^r, 5285 bp	This work
pMD18T-yjhG	Amp^r, carries yjhG, 6034 bp	This work
pMD18T-ΔyjhG	Amp^r, carries part of yjhG, Str^r, 5416 bp	This work
pMD18T-yagG	Amp^r, carries yagG gene, 5237 bp	This work
pMD18T-ΔyagG	Amp^r, carries part of yagG gene, Apr^r, 5897 bp	This work
pMD18T-xyL	Amp^r, carries β-xylosidase gene, 5642 bp	This work
pMD18T-ΔxyL	Amp^r, carries part of β-xylosidase gene, Apr^r, 6187 bp	This work
pMD18T-iclR	Amp^r, carries iclR, 4637 bp	This work
pMD18T-ΔiclR	Amp^r, carries part of iclR, Apr^r, 5228 bp	This work
pMD18T-yqhD	Amp^r, carries yqhD, 3856 bp	This work
pMD18T-ΔyqhD	Amp^r, carries part of yqhD, Str^r, 4268 bp	This work
pMD18T-betB	Amp^r, carries betB, 5308 bp	This work
pMD18T-ΔbetB	Amp^r, carries part of betB, Str^r, 5261 bp	This work
pIJ773	Apr^r, aac(3)IV with FRT sites, 4334 bp	[12]
pIJ778	Str^r, aadA with FRT sites, 4337 bp	[12]
pIJ790	Cm^r, encodes λ-Red genes, 6084 bp	[12]
pSARI	Kan^r, PR, 4,914 bp (Genbank MH037013)	Lab stock
pSARI-red	Kan^r, carries λ-Red genes, 6,799 bp	This work
pSARI-yqhD	Kan^r, carries the yqhD, 6078 bp	This work
pSARI-betB	Kan^r, carries the betB, 6630 bp	This work
Pet 28a	Vector carries N-terminal His Tag, Kanr, 5369 bp	Novagen^®
Pet 28a-yqhD	Amp^r, carries the yqhD, 6520 bp	This work
Pet 28a-aldB	Kan^r, carries the aldB, 6895 bp	This work
Pet 28a-betB	Kan^r, carries the betB, 6772 bp	This work
Pet 28a-ad1	Kan^r, carries the ad1, 6727 bp	This work
Pet 28a-ad2	Kan^r, carries the ad2, 6826 bp	This work

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ISSN: 1475-2859

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