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Table 1 Enzyme activities in the cell-free extracts of recombinant strains

From: Efficient production of (S)-1-phenyl-1,2-ethanediol using xylan as co-substrate by a coupled multi-enzyme Escherichia coli system

Strains

Specific activities (U/mg)

Towards 2-HAP

Towards xylose

Towards xylan

E. coli BL21/pET-SCRII

2.73 ± 0.01

ND

ND

E. coli BL21/pET-A258F/GDH

ND

6.85 ± 0.02

ND

E. coli BL21/pET-XYN2

ND

ND

267.51 ± 11.8

E. coli BL21/pET-S-G-2

1.54 ± 0.01

ND

65.13 ± 2.2

E. coli BL21/pET-S-2-G

1.28 ± 0.02

ND

54.45 ± 3.1

E. coli BL21/pET-G-S-2

0.85 ± 0.02

1.36 ± 0.01

55.33 ± 4.3

E. coli BL21/pET-G-2-S

ND

1.18 ± 0.02

35.93 ± 2.6

  1. ND not detected