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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Efficient production of (S)-1-phenyl-1,2-ethanediol using xylan as co-substrate by a coupled multi-enzyme Escherichia coli system

Fig. 5

The effects of temperature (a) and pH (b) on E. coli BL21/pET-G-S-2 catalyzing 2-HAP biotransformation. The temperature optimum of 2-HAP transformation was determined at various temperatures (20–50 °C). The pH optimum of 2-HAP transformation was determined between pH 3.0 and 10.0 using 0.1 M citric acid buffer (pH 3.0, 4.0, 5.0, 6.0, 6.5), 0.1 M potassium phosphate buffer (pH 6.5, 7.0, 7.5), and 50 mM Tris–HCl buffer (pH 8.0, 9.0, 10.0)

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Microbial Cell Factories

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