Improving cytosolic aspartate biosynthesis increases glucoamylase production in Aspergillus niger under oxygen limitation

Table 2 Kinetic parameters of mutant OE-asp2, mutant OE-asp1 and the parental strain CBS 513.88 in the 5 L fed-batch fermentation

	Strains	OE-asp1	OE-asp2	CBS 513.88
Batch phase	μ (h⁻¹)	0.062 ± 0.003	0.11 ± 0.02	0.07 ± 0.008
	q_GA (mg gDCW⁻¹ h⁻¹)	7.16 ± 0.01	7.10 ± 0.78	6.75 ± 0.13
	Y_p/x (g/g)	0.11 ± 0.006	0.09 ± 0.008	0.10 ± 0.009
Oxygen-limited phase	μ (h⁻¹)	0.012 ± 0.001	0.009 ± 0.002	0.013 ± 0.002
	q_GA (mg gDCW⁻¹ h⁻¹)	3.32 ± 0.07	5.56 ± 0.32	3.45 ± 0.15
	Y_p/x (g/g)	0.29 ± 0.02	0.61 ± 0.15	0.29 ± 0.02

In batch and oxygen-limited phases, μ and q_GA both represent the average values. Data in the table represent the average values and standard deviations from three replicates
μ specific growth rate. q_GA specific glucoamylase production rate, Y_p/x yield of glucoamylase based on biomass

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