Fig. 4

Intracellular amino acid pools of the parental strain CBS 513.88 (black line), mutant OE-asp1 (red line) and mutant OE-asp2 (blue line) at different time points: 36 h (exponential growth phase), 48 h (transition from batch phase to oxygen-limited phase), 66 h (oxygen-limited phase) and 85 h (oxygen-limited phase) in the 5 L fermentor. Data represent the average values and standard deviations from three individual experiments. The X-axis represents the fermentation time (h). The Y-axis represents the intracellular pools of amino acids (μmol/g DCW). The light yellow dotted box represents the Pentose Phosphate pathway that is not showed in detail. G6P glucose-6-phosphate, R5P ribose-5-phosphate, E4P erythrose-4-phosphate, F6P fructose-6-phosphate, 3PG 3-phosphoglycerate, PEP phosphoenolpyruvate, PYR-cyt cytosolic pyruvate, PYR-mito mitochondrial pyruvate, AcCoA acetyl-CoA, CIT citrate, IsCIT isocitrate, AKG 2-oxoglutarate, SUCCoA succinyl-CoA, SUC succinate, FUM fumarate, MAL malate, OAA-cyt cytosolic oxaloacetate, OAA-mito mitochondrial oxaloacetate, His histidine, Phe phenylalanine, Tyr tyrosine, Trp tryptophan, Ala alanine, Val valine, Leu leucine, Ile isoleucine, Glu glutamate, Lys lysine, Pro proline, Gln glutamine, Ser serine, Gly glycine, Cys cysteine, Asp aspartate, Asn asparagine, Met methionine, Thr threonine, Orn ornithine