Table 1 Strains used in this study
Name | Description | Source |
|---|---|---|
E. coli DH5α | E. coli strain for cloning | TIANGEN CO., LTD. |
M. neoaurum ATCC 25795 (Mn) | Wild type strain, the starting strain | ATCC |
MnΔkstD1 | kstD1 deleted in M. neoaurum ATCC 25795 | [6] |
WI | kstD1, kstD2 and kstD3 deleted in M. neoaurum ATCC 25795, 9-OHAD producing strain | [6] |
WIΔhadA | hadA deleted in WI strain | This study |
WIΔhadC | hadC deleted in WI strain | This study |
WIΔkasB | kasB deleted in WI strain | This study |
WIΔmmaAN | mmaAN (N represents 1, 2, 3, 4) deleted in WI strain | This study |
WIΔpks13 | pks13 deleted in WI strain | This study |
MnΔkasB | kasB deleted in M. neoaurum ATCC 25795 | This study |
MnΔkasB + kasB | kasB complemented in MnΔkasB strain | This study |
WIII | kshA1, kshA2, hsd4A, kstD1, kstD2 and kstD3 deleted in M. neoaurum ATCC 25795, 4-HBC producing strain | [7] |
WIIIΔkasB | kasB deleted in WIII strain | This study |