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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Disruption or reduced expression of the orotidine-5′-decarboxylase gene pyrG increases citric acid production: a new discovery during recyclable genome editing in Aspergillus niger

Fig. 4

Citric acid fermentation characteristics of pyrG titratable expression mutant. a Citric acid titer and b normalized citric acid titer (g citric acid/g dry weight) was detected and calculated for each Dox concentration. 1 × 105 spores/mL were inoculated in 20 mL citrate fermentation (CitFM) media with different Dox concentration and incubated at 34 °C in the dark for 96 h. The extracellular citric acid was determined by the method of HPLC. Pairwise Student’s t-tests were conducted between conditional expression mutant relative to the parent strains. p values are indicated as (< 0.05, *; < 0.01, **; < 0.001, ***)

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Microbial Cell Factories

ISSN: 1475-2859

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