Resolution of alkaloid racemate: a novel microbial approach for the production of enantiopure lupanine via industrial wastewater valorization

Table 1 Biocatalytic resolution of racemic mixtures

Biocatalyst	Racemic compound	Purified enantiomer	Initial racemate conc. (g L⁻¹)	Remaining racemate conc. (%)	ee (%)	References
P. putida LPK411	Lupanine	L-(−)-lupanine	1.23	39	99	Current study
R. rhodochrous LPK211	Lupanine	L-(−)-lupanine	1.45	60	100	Current study
Rhodococcus sp. LPK311	Lupanine	L-(−)-lupanine	1.39	60	96	Current study
P. putida (NCIB9494)	Carvedilol	(S)-(−)-carvedilol	0.25	34.92	95	Ettireddy et al. [22]
Rhodococcus sp. CCZU10-1	Phenyl methyl sulfoxide	(S)-phenyl methyl sulfoxide	14.02	55.3	> 99.9	He et al. [23]
P. putida (NBRC 14164) ω-transaminase	α-methylbenzylamine	(R)-α-methylbenzylamine	6.06	49.8	> 99.0	Wu et al. [24]
P. putida (NBRC 14164) ω-transaminase	2-amino-2-phenylethanol	(S)-2-amino-2-phenylethanol	5.49	49.3	> 99.0	Wu et al. [24]
R. opacus L-AAO oxidase	Leucine	d-Leucine	0.91	50	> 99.2	Geueke and Hummel [25]
R. opacus L-AAO oxidase	Phenylalanine	d-Phenylalanine	1.16	50	> 99.5	Geueke and Hummel [25]

ISSN: 1475-2859