Fig. 4

Effect of DR1558 on cell growth, glucose consumption, glutamate production, and gamma aminobutyric acid (GABA) production in the recombinant Escherichia coli. The E. coli DGB202 and DGB203 strains were cultured in MR medium supplemented with 5 g/L yeast extract, 30 g/L glucose, and 15 g/L ammonium sulfate for 24 h. The E. coli DGB302 and DGB303 strains were cultured in MR medium supplemented with 5 g/L yeast extract, 20 g/L glucose, and 10 g/L ammonium sulfate for 48 h. a Cell growth; b glucose consumption; c glutamate production; d GABA production. Histogram shows the mean of three biological replicates and error bars represent standard deviation