Fig. 2
From: Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli
Representative DNA sequencing results of recombination derivatives from pDG-A-X series. MUT-1 had deletion of the first gRNA expression cassette; MUT-2 had deletion of the second gRNA expression cassette; MUT-3 and MUT-4 had point mutations in the second promoter J23119; MUT-5 had 12-bp repeated insertion in the second gRNA scaffold. Promoter J23119 (purple), 82-bp scaffold (blue) and the mutated sequence (highlighted) are denoted. The strikeout represents deleted sequence