Table 4 The ribosome binding sites used in this study
From: Expression regulation of multiple key genes to improve l-threonine in Escherichia coli
Names | Sequence (5′-3′) | RBS Calculator’s proportional scale (au) [64] |
|---|---|---|
RBSthrL | ATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCC | – |
RBSthrA | CGACCAAAGGTAACGAGGTAACAACC | – |
RBSiclR | ACGATACAGAAAAAAGAGACTGTC | – |
RBSs1 | GCTCAGAAAAATAAGAAGAGGTATCTCA | 3800 |
RBSs2 | ACCAAAACCATAAGGAGGATTTAC | 380,000 |
RBSs3 | GTAGCTGAGACTCTAAGGGAGGCGTCAC | 45000 |
RBSs4 | GCATACATTCCACGACCATAGGAACCAACCC | 15,000 |
RBSs5 | AAGCCCATTTTACCATCGAAGAGGGACAGA | 5000 |
RBSs6 | TCCTTTTAGGGCTATCGCGGAGGAGTA | 1000 |
RBSs7 | TAGCCACCTAGGAGGACTCC | 2000 |
RBSs8 | CGCATAACAATTCGTATCATAAGGAGACCAT | 2000 |
RBSs9 | AAGGCCAAAGACACTAAGAATTATTTAT | 2000 |
RBSarcA | TACTTCCTGTTTCGATTTAGTTGGCAATTTAGGTAGCAAAC | – |
RBScpxR | CGGAGGTATTTAAACA | – |
RBSgadE | ATAACGGCTAAGGAGCAAGTT | – |
RBSpykF | CTCCCATCCTTCTCAACTTAAAGACTAAGACTGTC | – |
RBSfadR | TGCTGTGTTATGGAAATCTCACT | – |