Table 1 Bacterial trains and plasmids used in this study
From: Expression regulation of multiple key genes to improve l-threonine in Escherichia coli
Strains or plasmids | Description | Sources |
|---|---|---|
Strains | ||
JM109 | Wild type E. coli | NEB |
MG1655 | Wild type E. coli K-12; F−λ− rph-1 | CGSC 6300 |
TWF001 | l-Threonine-producing E. coli strain | [9] |
TWF003 | TWF001ΔiclR | [9] |
TWF010 | TWF001ΔarcA | [46] |
TWF051 | Derived from TWF001 by replacing RBSarcA (the RBS sequence of arcA) with thrR (RBSthrL-thrL-RBSthrA, the RBS sequence of thrL connected with the DNA sequence of the thr operon leader regions, thrL, and followed by the RBS sequence of thrA) | This study |
TWF052 | Derived from TWF001 by replacing RBScpxR with thrR | This study |
TWF053 | Derived from TWF001 by replacing RBSgadE with thrR | This study |
TWF054 | Derived from TWF001 by replacing RBSpykF with thrR | This study |
TWF055 | Derived from TWF001 by replacing RBSfadR with thrR | This study |
TWF056 | Derived from TWF001 by inserting the DNA sequence thrR1 (the DNA sequence of the thr operon leader regions, thrL, followed by the RBS sequence of thrA) between RBSiclR (the RBS sequence of iclR) and gene iclR | This study |
TWF057 | Derived from TWF001 by inserting the DNA sequence thrR2 (the RBS sequence of thrL, followed by thrL) between PiclR (the Promoter sequence of iclR) and RBSiclR | This study |
TWF058 | Derived from TWF001 by replacing RBSiclR with the DNA sequence thrR | This study |
TWF059 | Derived from TWF001 by replacing PiclR and RBSiclR with the DNA sequence Pthrl-thrR | This study |
TWF060 | Derived from TWF058 by replacing RBSiclR with thrRs1 (RBSthrL-thrL-RBSs1) | This study |
TWF061 | Derived from TWF058 by replacing RBSiclR with thrRs2 (RBSs2-thrL-RBSthrA) | This study |
TWF062 | Derived from TWF058 by replacing RBSiclR with thrRs3 (RBSs3-thrL-RBSthrA) | This study |
TWF063 | Derived from TWF058 by replacing RBSiclR with thrRs4 (RBSs4-thrL-RBSthrA) | This study |
TWF064 | Derived from TWF058 by replacing RBSiclR with thrRs5 (RBSs5-thrL-RBSthrA) | This study |
TWF065 | Derived from TWF058 by replacing RBSiclR with thrRs6 (RBSs6-thrL-RBSthrA) | This study |
TWF066 | Derived from TWF063 by inserting the DNA sequence PcysH-RBSs7-aspC (the Promoter sequence of cysH followed by a synthetic RBSs7 and the gene aspC) in the lacI locus | This study |
TWF067 | Derived from TWF063 by inserting a DNA sequence PcysJ-RBSs8-aspC in the lacI locus | This study |
TWF068 | Derived from TWF063 by inserting a DNA sequence PcysD-RBSs9-aspC in the lacI locus | This study |
TWF069 | Derived from TWF066 by replacing RBSarcA with thrR | This study |
TWF070 | Derived from TWF066 by replacing RBScpxR with thrR | This study |
TWF071 | Derived from TWF066 by replacing RBSgadE with thrR | This study |
TWF072 | Derived from TWF066 by replacing RBSpykF with thrR | This study |
TWF073 | Derived from TWF066 by replacing RBSfadR with thrR | This study |
TWF074 | Derived from TWF070 by replacing RBSarcA with thrR | This study |
TWF075 | Derived from TWF070 by replacing RBSgadE with thrR | This study |
TWF076 | Derived from TWF070 by replacing RBSpykF with thrR | This study |
TWF077 | Derived from TWF070 by replacing RBSfadR with thrR | This study |
TWF078 | Derived from TWF077 by replacing RBSarcA with thrR | This study |
TWF079 | Derived from TWF077 by replacing RBSgadE with thrR | This study |
TWF080 | Derived from TWF077 by replacing RBSpykF with thrR | This study |
TWF081 | Derived from TWF078 by replacing RBSgadE with thrR | This study |
TWF082 | Derived from TWF078 by replacing RBSpykF with thrR | This study |
TWF083 | Derived from TWF081 by replacing RBSpykF with thrR | This study |
Plasmids | ||
pCas9 | repA101(Ts) kan Pcas-cas9 ParaB-Red lacIq Ptrc-sgRNA-pMB1 | [49] |
pTargetF | pMB1 aadA sgRNA | [49] |
pTargetF-arcA | pMB1 aadA sgRNA-arcA | This study |
pTargetF-cpxR | pMB1 aadA sgRNA- | This study |
pTargetF-gadE | pMB1 aadA sgRNA-gadE | This study |
pTargetF-pykF | pMB1 aadA sgRNA-pykF | This study |
pTargetF-fadR | pMB1 aadA sgRNA-fadR | This study |
pTargetF-iclR1 | pMB1 aadA sgRNA-iclR1 | This study |
pTargetF-iclR2 | pMB1 aadA sgRNA-iclR2 | This study |
pTargetF-lacI | pMB1 aadA sgRNA-lacI | This study |