Skip to main content
Fig. 1 | Microbial Cell Factories

Fig. 1

From: Novel plasmid-free Gluconobacter oxydans strains for production of the natural sweetener 5-ketofructose

Fig. 1

Scheme of the loci used for targeted integration of the fdhSCL genes into the genome of G. oxydans IK003.1. Shown are the integration sites with the flanking genes, the P264 promoter (black arrow), a consensus RBS (black rectangle), the fdhSCL genes (gray arrows), and terminators (black hairpin = native, gray hairpin = inserted). The transcription strength of the flanking genes (strong, medium, weak) and the normalized read count (given by Fragments Per Kilobase of transcript per Million mapped reads (FPKM) values in brackets) are also indicated. FPKM values were determined via RNAseq with G. oxydans 621H grown on mannitol (exponential growth phase) [33]. To differentiate the transcription levels, the FPKM values (range of 0–446,627) were grouped in the lowest 25% (0–38, weak), the median 50% (38–318, medium) and the highest 25% (318–446,627, strong)

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us