Table 2 The plasmids used in this work
Name | Relative characteristics | Source |
|---|---|---|
pUC19 | TaKaRa | |
pTAs-HUH | HisG-Ura3-HisG maker recycled cassette | [36] |
pCAS1yl | TRP1 Guide RNA module and Cas9 expression cassette in pMCSCen1 | [36] |
pCAS1yl-ku70 | Ku70 Guide RNA module and Cas9 expression cassette in pMCSCen1 | This work |
pCAS1yl-ku80 | Ku80 Guide RNA module and Cas9 expression cassette in pMCSCen1 | This work |
pCAS1yl-rDNA | rDNA Guide RNA module and Cas9 expression cassette in pMCSCen1 | This work |
pCAS1yl-D17 | D17 Guide RNA module and Cas9 expression cassette in pMCSCen1 | This work |
pCAS1yl-lip1 | Lip1 Guide RNA module and Cas9 expression cassette in pMCSCen1 | This work |
pCAS1yl-pox3 | Pox3 Guide RNA module and Cas9 expression cassette in pMCSCen1 | This work |
pCAS1yl-pox4 | Pox4 Guide RNA module and Cas9 expression cassette in pMCSCen1 | This work |
pCAS1yl-pox5 | Pox5 Guide RNA module and Cas9 expression cassette in pMCSCen1 | This work |
pCAS1yl-LEU2 | LEU2 Guide RNA module and Cas9 expression cassette in pMCSCen1 | This work |
pCAS1yl-XPR2 | XPR2 Guide RNA module and Cas9 expression cassette in pMCSCen1 | This work |
pUC19-TBX | PTEF1-CarB-xpr2t cassette in pUC19 | This work |
pUC19-ERPL | PEXP1-CarRP-lip2t cassette in pUC19 | This work |
pUC19-GCM | PGPD2-CCD1-mig1t cassette in pUC19 | This work |
pUC19-TGX | PTEF1-GGS1-xpr2t cassette in pUC19 | This work |
pUC19-GTM | PEXP1-tHMG1-lip2t cassette in pUC19 | This work |
pUC19-rDNA-BPC | PTEF1-CarB-xpr2t and PEXP1-CarRP-lip2t and PGPD2-CCD1-mig1t cassettes in pUC19-rDNA-HUH | This work |
pUC19-ku70-BPC | PTEF1-CarB-xpr2t and PEXP1-CarRP-lip2t and PGPD2-CCD1-mig1t cassettes in pUC19-ku70-HUH | This work |
pUC19-ku80-GGS1-HUH | PTEF1-GGS1-xpr2t cassette in pUC19-ku80-HUH | This work |
pUC19-D17-tHMG1-HUH | PEXP1-tHMG1-lip2t cassette in pUC19-D17-HUH | This work |
pUC19-lip1-ERG10ERG13-HUH | PTEF1-ERG10-erg10t and PGPD2-ERG13-erg13t cassettes in pUC19-lip1-HUH | This work |
pUC19-POX3-IDIERG20-HUH | PEXP1-IDI-idit and PTEF1-ERG20-erg20t cassettes in pUC19-pox3-HUH | This work |
pUC19-POX5-ERG8ERG12ERG19-HUH | PEXP1-ERG8-erg8t, PTEF1-ERG12-erg12t and PGPD2-ERG19-erg19t cassettes in pUC19-pox5-HUH | This work |
pUC19-rDNA-BB-PK–PTA-HUH | PGPD2-B. b pk-mig1t-PTEF1-B. s pta-xpr2t cassettes in pUC19-rDNA-HUH | This work |
pUC19-rDNA-BC-PK–PTA-HUH | PGPD2-B. b pk-mig1t-PTEF1-C. k pta-xpr2t cassettes in pUC19-rDNA-HUH | This work |
pUC19-rDNA-LB-PK–PTA-HUH | PGPD2-L. m pk-mig1t-PTEF1-B. s pta-xpr2t cassettes in pUC19-rDNA-HUH | This work |
pUC19-rDNA-LC-PK–PTA-HUH | PGPD2-L. m pk-mig1t-PTEF1-C. k pta-xpr2t cassettes in pUC19-rDNA-HUH | This work |
pUC19-POX4-BB-PK–PTA-HUH | PGPD2-B. b pk-mig1t-PTEF1-B. s pta-xpr2t cassettes in pUC19-POX4-HUH | This work |
pUC19-XPR2-BB-PK–PTA-HUH | PGPD2-B. b pk-mig1t-PTEF1-B. s pta-xpr2t cassettes in pUC19-XPR2-HUH | This work |