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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone

Fig. 1

Cloning and recombination of pGH. a Cloning of pGH genes (GenBank: X53325). Using the cDNA of pGH gene obtained after the reverse transcription of total cerebellum RNA as a template, the pGH full-length gene was cloned by amplification of pGH gene primer. Lane 1, EcoRI and NotI amplification product with enzyme cleavage site, lane 2, XhoI and XbaI amplification product with enzyme cleavage site, lane M, DL2000 standard molecular weight DNA Marker. b Construction recombination of pPICZA–pGH. pPICZA–pGH was linearized by SacI, to transformed into Pichia pastoris X33, which was screened by Zeocin, and the colony PCR was performed with general primers 5′ AOX1 and 3′ AOX1. Lanes 1–5, the intracellular expression of pGH in Pichia pastoris X33, lane M, DL2000 standard molecular weight DNA Marker

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Microbial Cell Factories

ISSN: 1475-2859

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