Fig. 2
Expression levels of the bus genes in S. pogona and mutant strains. mRNA samples were isolated from wild-type and engineering strain cells cultured for 144 h, The transcriptional levels (log10) of the genes busA, busC, busD, busE, busF, busG, busI, busP, busO, busK and busS genes in S. pogona-Sp1418 were 0.39-, 1.12-, 0.51-, 0.48-, 0.60-, 1.06-, 1.57-, 1.89-, 0.14-, 0.36-, and 0.47-fold higher than those in S. pogona, respectively, which indicated a significant upregulation. Limited expression was detected for many bus genes in, and only busB, busF, busI, busP, and busS were detected, with expression levels (log10) of 0.05, 0.65, 0.96, 0.96 and 0.67 times that of wild-type bacteria. The 16S rRNA gene was used as an internal control to quantify the relative expression of target genes. Gene expression differences are shown by bar height. Error bars represent the standard deviation of the mean. *, **and *** indicated P < 0.05, P < 0.01 and P < 0.005, respectively, compared to S. pogona under the same conditions. Error bars indicated standard errors of results from n = 3 replicates