A secretion biosensor for monitoring Sec-dependent protein export in Corynebacterium glutamicum

Table 1 C. glutamicum genes responding to Sec secretion stress in microarray experiments

Gene locus	Gene	Annotation	Average mRNA ratio ΔSP-AmyE overproduction^a	Average mRNA ratio AmyE overproduction^b	Average mRNA ratio NrpE-cutinase overproduction^c
cg0998	htrA	HtrA protease homologue	1.24	4.22	4.40
cg0706	esrI	EsrI, inhibitor of the EsrSR two-component system	0.99*	2.31	0.80*
cg1325		Putative stress responsive transcriptional regulator	1.13*	3.69	0.96*
cg3264	rsmP	RsmP, conserved cytoskeletal protein	0.61	2.67	0.96*

Significant (p ≤ 0.05) more than twofold changes in the average mRNA ratios in the DNA microarray experiments are indicated in italic numbers. Average mRNA ratios with p > 0.05 are marked by an asterisk. All microarray data were from three independent biological samples
^aAverage mRNA ratio of C. glutamicum (pEKEx2-ΔSP-AmyE) induced/uninduced by IPTG for 30 min
^bAverage mRNA ratio of C. glutamicum (pEKEx2-AmyE) induced/uninduced by IPTG for 30 min
^cAverage mRNA ratio of C. glutamicum (pEKEx2-NprE-cutinase) induced/uninduced by IPTG for 30 min

ISSN: 1475-2859