Fig. 7

Production of α-acetolactate from glucose via pyruvate. Glucose is fermented using FS1072 in M17 broth and pyruvate is obtained (Phase I). After 74 h FS1072 was removed from the broth and RD06 was applied as whole cell biocatalyst for converting pyruvate into α-acetolactate (Phase II). The concentrations of glucose (squares), pyruvate (triangles), α-acetolactate (diamonds) and acetoin (inverted triangle) are shown [mM]. Lactate and acetate were observed at different levels after phase I (81 ± 8 mM and 38 ± 8 mM, respectively), but they remained constant during phase II and are therefore not shown